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FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS

A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-label...

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Bibliografiske detaljer
Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Gall, Joseph G., Pardue, Mary Lou
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1969
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC223575/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4895535/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.63.2.378
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