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Video fluorescence microscopy studies of phospholipid vesicle fusion with a planar phospholipid membrane. Nature of membrane-membrane interactions and detection of release of contents

Video fluorescence microscopy was used to study adsorption and fusion of unilamellar phospholipid vesicles to solvent-free planar bilayer membranes. Large unilamellar vesicles (2-10 microns diam) were loaded with 200 mM of the membrane-impermeant fluorescent dye calcein. Vesicles were ejected from a...

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Библиографические подробности
Формат: Artigo
Язык:Inglês
Опубликовано: The Rockefeller University Press 1987
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2228874/
https://ncbi.nlm.nih.gov/pubmed/3694175
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