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Evaluations of Different Hypervariable Regions of Archaeal 16S rRNA Genes in Profiling of Methanogens by Archaea-Specific PCR and Denaturing Gradient Gel Electrophoresis

Different hypervariable (V) regions of the archaeal 16S rRNA gene (rrs) were compared systematically to establish a preferred V region(s) for use in Archaea-specific PCR-denaturing gradient gel electrophoresis (DGGE). The PCR products of the V3 region produced the most informative DGGE profiles and...

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Bibliografische gegevens
Hoofdauteurs: Yu, Zhongtang, García-González, Rubén, Schanbacher, Floyd L., Morrison, Mark
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 2008
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2227698/
https://ncbi.nlm.nih.gov/pubmed/18083874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00684-07
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