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Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)
A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using p...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2006
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2225213/ https://ncbi.nlm.nih.gov/pubmed/17077498 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106041959 |
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