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Expression, purification and crystallization of a PCI domain from the COP9 signalosome subunit 7 (CSN7)

A core fragment of Arabidopsis thaliana COP9 signalosome (CSN) subunit 7 was expressed in Escherichia coli. The protein was purified to homogeneity and screened for crystallization. Crystallization conditions were refined using the sitting-drop vapour-diffusion method. Crystals were obtained using p...

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Detalhes bibliográficos
Main Authors: Dessau, Moshe, Chamovitz, Daniel A., Hirsch, Joel A.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225213/
https://ncbi.nlm.nih.gov/pubmed/17077498
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106041959
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