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THE DIRECT ISOLATION OF THE MITOTIC APPARATUS

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releas...

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Bibliografiska uppgifter
Huvudupphovsmän: Mazia, Daniel, Mitchison, J. M., Medina, Heitor, Harris, Patricia
Materialtyp: Artigo
Språk:Inglês
Publicerad: The Rockefeller University Press 1961
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2225108/
https://ncbi.nlm.nih.gov/pubmed/13768661
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