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Combined Real-Time PCR and Pyrosequencing Strategy for Objective, Sensitive, Specific, and High-Throughput Identification of Reduced Susceptibility to Penicillins in Neisseria meningitidis

A segment of penA in Neisseria meningitidis strains (n = 127), including two nucleotide sites closely associated to reduced susceptibility to penicillins, was amplified and pyrosequenced. All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pe...

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Autors principals: Thulin, Sara, Olcén, Per, Fredlund, Hans, Unemo, Magnus
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2008
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2224723/
https://ncbi.nlm.nih.gov/pubmed/18070955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00914-07
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