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Folding of a large ribozyme during transcription and the effect of the elongation factor NusA
We compared in vitro transcription-initiated folding of the ribozyme from Bacillus subtilis RNase P to refolding from the full-length, denatured state by monitoring the appearance of its catalytic activity. At 37°C, Mg(2+)-initiated refolding of the wild type and a circularly permutate ribozyme take...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
The National Academy of Sciences
1999
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC22245/ https://ncbi.nlm.nih.gov/pubmed/10449729 |
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