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Folding of a large ribozyme during transcription and the effect of the elongation factor NusA

We compared in vitro transcription-initiated folding of the ribozyme from Bacillus subtilis RNase P to refolding from the full-length, denatured state by monitoring the appearance of its catalytic activity. At 37°C, Mg(2+)-initiated refolding of the wild type and a circularly permutate ribozyme take...

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Detalhes bibliográficos
Main Authors: Pan, Tao, Artsimovitch, Irina, Fang, Xing-wang, Landick, Robert, Sosnick, Tobin R.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22245/
https://ncbi.nlm.nih.gov/pubmed/10449729
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