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Functional Coupling of Last-Intron Splicing and 3′-End Processing to Transcription In Vitro: the Poly(A) Signal Couples to Splicing before Committing to Cleavage

We have developed an in vitro transcription system, using HeLa nuclear extract, that supports not only efficient splicing of a multiexon transcript but also efficient cleavage and polyadenylation. In this system, both last-intron splicing and cleavage/polyadenylation are functionally coupled to tran...

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Main Authors: Rigo, Frank, Martinson, Harold G.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2223410/
https://ncbi.nlm.nih.gov/pubmed/17967872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.01410-07
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