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Crystallization and preliminary X-ray analysis of the C-terminal RNase III domain of human Dicer

Human Dicer protein contains two RNase III domains (RNase IIIa and RNase IIIb) which are involved in the production of short interfering RNAs (siRNAs). The C-terminal RNase III domain (RNase IIIb) of human Dicer was expressed, purified and crystallized by the sitting-drop vapour-diffusion method. Th...

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Bibliografiske detaljer
Main Authors: Takeshita, Daijiro, Zenno, Shuhei, Lee, Woo Cheol, Nagata, Koji, Saigo, Kaoru, Tanokura, Masaru
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2222580/
https://ncbi.nlm.nih.gov/pubmed/16582496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910600889X
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