Crystallization and preliminary X-ray analysis of the tRNA thiolation enzyme MnmA from Escherichia coli complexed with tRNA(Glu)

MnmA catalyzes a sulfuration reaction to synthesize 2-thiouridine at the wobble positions of tRNA(Glu), tRNA(Gln) and tRNA(Lys) in Escherichia coli. The binary complex of MnmA and tRNA(Glu) was crystallized in two different crystal forms: forms I and II. Cocrystallization of MnmA–tRNA(Glu) with ATP...

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Bibliographic Details
Main Authors: Numata, Tomoyuki, Ikeuchi, Yoshiho, Fukai, Shuya, Adachi, Hiroaki, Matsumura, Hiroyoshi, Takano, Kazufumi, Murakami, Satoshi, Inoue, Tsuyoshi, Mori, Yusuke, Sasaki, Takatomo, Suzuki, Tsutomu, Nureki, Osamu
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2006
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2222564/
https://ncbi.nlm.nih.gov/pubmed/16582487
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910600738X
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