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Thymidine 5′-Monophosphate-Requiring Mutants of Saccharomyces cerevisiae Are Deficient in Thymidylate Synthetase

Thymidylate synthetase activity was measured in crude extracts of the yeast Saccharomyces cerevisiae by a sensitive radiochemical assay. Spontaneous non-conditional mutants auxotrophic for thymidine 5′-monophosphate (tmp1) lacked detectable thymidylate synthetase activity in cell-free extracts. In c...

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Detalhes bibliográficos
Main Authors: Bisson, Linda, Thorner, Jeremy
Formato: Artigo
Idioma:Inglês
Publicado em: 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC221824/
https://ncbi.nlm.nih.gov/pubmed/334734
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