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Isolation of conditional lethal mutator mutants of Escherichia coli by localized mutagenesis.

By using localized mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine, we isolated 39 temperature-sensitive growth mutants that exhibited high mutability when the bacteria were grown at the permissive temperature. Two of the mutations, dnaQ186 and dnaQ231, were shown to be new alleles of the...

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Bibliographic Details
Main Authors: Maki, H, Horiuchi, T, Sekiguchi, M
Format: Artigo
Language:Inglês
Published: 1983
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC221786/
https://ncbi.nlm.nih.gov/pubmed/6337996
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