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Functional Reconstitution of a Prokaryotic K(+) Channel

SliK, a K(+) channel encoded by the Streptomyces KcsA gene, was expressed, purified, and reconstituted in liposomes. A concentrative (86)Rb(+) flux assay was used to assess the ion transport properties of SliK. SliK-mediated ionic flux shows strong selectivity for K(+) over Na(+) and is inhibited by...

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Detalhes bibliográficos
Main Authors: Heginbotham, Lise, Kolmakova-Partensky, Ludmila, Miller, Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2217152/
https://ncbi.nlm.nih.gov/pubmed/9607934
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