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RecA polymerization on double-stranded DNA by using single-molecule manipulation: The role of ATP hydrolysis

The polymerization of RecA on individual double-stranded DNA molecules is studied. A linear DNA (λ DNA, 48.5 Kb), anchored at one end to a cover glass and at the other end to an optically trapped 3-μm diameter polystyrene bead, serves as a template. The elongation caused by RecA assembly is measured...

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Detalhes bibliográficos
Main Authors: Shivashankar, G. V., Feingold, M., Krichevsky, O., Libchaber, A.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC22162/
https://ncbi.nlm.nih.gov/pubmed/10393922
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