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Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.

Salmonella phage P22 was utilized as a vector for phage Mu cts d1(Apr lac) mutagenesis in Salmonella typhimurium. Efficient transposition of phage Mu d1 and the construction of gene fusions were readily accomplished with this procedure. Mutants blocked in the biosynthesis of NAD+ and in pyridine nuc...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Holley, E A, Foster, J W
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1982
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221562/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6752128/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.152.2.959-962.1982
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