Cloning of and complementation tests with alkaline phosphatase regulatory genes (phoS and phoT) of Escherichia coli.

The regulatory genes of alkaline phosphatase, phoS and phoT, of Escherichia coli were cloned on pBR322, initially as an 11.8-kilobase EcoRI fragment. A restriction map of the hybrid plasmid was established. Deletion plasmids of various sizes were constructed in vitro, and the presence of phoS and ph...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Amemura, M, Shinagawa, H, Makino, K, Otsuji, N, Nakata, A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1982
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221518/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6290447/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.152.2.692-701.1982
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