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Cloning and identification of the product of the dnaE gene of Escherichia coli.

We successively subcloned the dnaE gene of Escherichia coli into pBR322, resulting in a plasmid that contains 4.6 kilobases of E. coli DNA. This plasmid can complement a dnaE temperature-sensitive mutation. A restriction map of the dnaE gene and the surrounding 10.7-kilobase region of the E. coli ch...

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Published in:J Bacteriol
Main Authors: Welch, M M, McHenry, C S
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1982
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC221416/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6288664/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.152.1.351-356.1982
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