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Enhanced Background Rejection in Thick Tissue with Differential-Aberration Two-Photon Microscopy
When a two-photon excited fluorescence (TPEF) microscope is used to image deep inside tissue, out-of-focus background can arise from both ballistic and nonballistic excitation. We propose a solution to largely reject TPEF background in thick tissue. Our technique is based on differential-aberration...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2212671/ https://ncbi.nlm.nih.gov/pubmed/17951295 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.111476 |
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