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Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensor
Periplasmic expression screening is a selection technique used to enrich high-affinity proteins in Escherichia coli. We report using this screening method to rapidly select a mutated D-glucose/D-galactose-binding protein (GGBP) having low affinity to glucose. Wild-type GGBP has an equilibrium dissoc...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2211708/ https://ncbi.nlm.nih.gov/pubmed/17905834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.073119507 |
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