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Rapid site-directed domain scanning mutagenesis of enteropathogenic Escherichia coli espD
We developed a rapid mutagenesis method based on a modification of the QuikChange® system (Stratagene) to systemically replace endogenous gene sequences with a unique similar size sequence tag. The modifications are as follows: 1: the length of the anchoring homologous sequences of both mutagenesis...
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| Huvudupphovsmän: | , , |
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| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
Biological Procedures Online
2007
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2211572/ https://ncbi.nlm.nih.gov/pubmed/18213361 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1251/bpo130 |
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