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Cloning of antibiotic resistance and nutritional genes in streptomycetes.

Methodology which allows consistent shotgun cloning of streptomycete genes is presented. Parameters that increase transformation efficiency of Streptomyces lividans 66 were adjusted to generate reproducibly a population of cloned genes likely to represent the entire genome. Factors which influence t...

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Detalhes bibliográficos
Main Authors: Thompson, C J, Ward, J M, Hopwood, D A
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC220307/
https://ncbi.nlm.nih.gov/pubmed/6284706
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