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Cloning of antibiotic resistance and nutritional genes in streptomycetes.

Methodology which allows consistent shotgun cloning of streptomycete genes is presented. Parameters that increase transformation efficiency of Streptomyces lividans 66 were adjusted to generate reproducibly a population of cloned genes likely to represent the entire genome. Factors which influence t...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Thompson, C J, Ward, J M, Hopwood, D A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1982
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC220307/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6284706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.151.2.668-677.1982
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