Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.
Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1982
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC220260/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7045080/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.151.1.462-464.1982 |
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