Genetic transformation of Rhizobium leguminosarum by plasmid DNA.

We demonstrated the genetic transformation of Rhizobium leguminosarum by R68.45 plasmid DNA by freezing and thawing cell suspensions in the presence of R68.45 plasmid DNA and 20 mM MgCl2. Clones resistant to kanamycin and tetracycline were recovered at a frequency of 10(-8) per recipient cell. No co...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Bullerjahn, G S, Benzinger, R H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1982
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC220134/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7061403/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.150.1.421-424.1982
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