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THE ISOLATION OF MOUSE HEPATOCYTE GAP JUNCTIONS : Preliminary Chemical Characterization and X-Ray Diffraction

A method is reported for isolating a preparation of hepatic gap junctions from the mouse. The method involves a collagenase digestion, treatment with the detergent Sarkosyl NL-97, and ultrasonication, followed by sucrose gradient ultracentrifugation. A run with 36 animals yields 0.1–0.5 mg protein....

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Hlavní autoři: Goodenough, Daniel A., Stoeckenius, Walther
Médium: Artigo
Jazyk:Inglês
Vydáno: The Rockefeller University Press 1972
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2200277/
https://ncbi.nlm.nih.gov/pubmed/4339819
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