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Reconstitution of nuclear protein transport with semi-intact yeast cells
We have developed an in vitro nuclear protein import reaction from semi- intact yeast cells. The reaction uses cells that have been permeabilized by freeze-thaw after spheroplast formation. Electron microscopic analysis and antibody-binding experiments show that the nuclear envelope remains intact b...
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| Format: | Artigo |
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| Sprache: | Inglês |
| Veröffentlicht: |
The Rockefeller University Press
1993
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2200159/ https://ncbi.nlm.nih.gov/pubmed/8227140 |
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