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Large-scale purification and crystallization of the endoribonuclease XendoU: troubleshooting with His-tagged proteins

XendoU is the first endoribonuclease described in higher eukaryotes as being involved in the endonucleolytic processing of intron-encoded small nucleolar RNAs. It is conserved among eukaryotes and its viral homologue is essential in SARS replication and transcription. The large-scale purification an...

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Detalhes bibliográficos
Main Authors: Renzi, Fabiana, Panetta, Gianna, Vallone, Beatrice, Brunori, Maurizio, Arceci, Massimo, Bozzoni, Irene, Laneve, Pietro, Caffarelli, Elisa
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2197201/
https://ncbi.nlm.nih.gov/pubmed/16511328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309106006373
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