Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.

The glutamine synthetase (GS) gene glnA of Thiobacillus ferrooxidans was cloned on recombinant plasmid pMEB100 which enabled Escherichia coli glnA deletion mutants to utilize (NH4)2SO4 as the sole source of nitrogen. High levels of GS-specific activity were obtained in the E. coli glnA deletion muta...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Barros, M E, Rawlings, D E, Woods, D R
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219347/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2866177/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.3.1386-1389.1985
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