Cloning and DNA sequence of a plasmid-determined citrate utilization system in Escherichia coli.

The citrate utilization determinant from a large 200-kilobase (kb) naturally occurring plasmid was previously cloned into the PstI site of plasmid vector pBR325 creating the Cit+ tetracycline resistance plasmid pWR61 (15 kb). Tn5 insertion mutagenesis analysis of plasmid pWR61 limited the segment re...

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Bibliographic Details
Main Authors: Sasatsu, M, Misra, T K, Chu, L, Laddaga, R, Silver, S
Format: Artigo
Language:Inglês
Published: 1985
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC219288/
https://ncbi.nlm.nih.gov/pubmed/2999088
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