Suicide vector for transposon mutagenesis in Pseudomonas solanacearum.

A suicide vector was constructed by cloning the transfer genes of the wide-host-range (IncW group) plasmid R388 into the BamHI site of pBR325. This plasmid can deliver Tn5 into Pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient.

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Bibliographic Details
Main Authors: Morales, V M, Sequeira, L
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC219269/
https://ncbi.nlm.nih.gov/pubmed/2993241
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