Mu-lac insertion-directed mutagenesis in a pectate lyase gene of Erwinia chrysanthemi.
The pelC gene, which encodes one of the five major pectate lyase (PL) isoenzymes in Erwinia chrysanthemi 3937, designated PLc, was subcloned from a hybrid lambda phage into a pBR322 derivative and mutagenized with a mini-Mu-lacZ transposable element able to form fusions to the lacZ gene. One plasmid...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1985
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219219/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2993251/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.163.3.913-917.1985 |
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