Cloning and mapping of the genetic determinants for microcin B17 production and immunity.
Plasmid pMccB17 (70 kilobases [kb]) codes for the production of microcin B17, a peptide that inhibits DNA synthesis, and for microcin B17 immunity. A BamHI-EcoRI fragment of 5.1 kb from pMccB17 was cloned into pBR322 in two steps. The resulting plasmid (pMM102) overproduced microcin B17 and expresse...
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| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1985
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219109/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989247/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.163.1.275-281.1985 |
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