Molecular cloning of the structural gene coding for CTP:CMP-3-deoxy-manno-octulosonate cytidylyltransferase from Escherichia coli K-12.
The kdsB gene from Escherichia coli K-12, which encodes CTP:CMP-3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase), was cloned into pBR322 as an 8-kilobase PstI fragment. Selection of this cloned segment was facilitated by using Salmonella typhimurium SL5283, which is deficient in...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219106/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2989246/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.163.1.256-261.1985 |
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