High-frequency transformation of Brevibacterium lactofermentum protoplasts by plasmid DNA.

An efficient polyethylene glycol-assisted method for transformation of Brevibacterium lactofermentum protoplasts that uses plasmid vectors has been developed. Two small plasmids, pUL330 (5.2 kilobases) and pUL340 (5.8 kilobases), both containing the kanamycin resistance gene from transposon Tn5 and...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Santamaria, R I, Gil, J A, Martin, J F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1985
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219017/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3980445/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.1.463-467.1985
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