Use of transcriptional repressors to stabilize plasmid copy number of transcriptional fusion vectors.
Strong promoters cloned into transcriptional fusion vectors can adversely affect plasmid copy number. In this study, we investigated the use of transcriptional repressors, lacI and tetR, to stabilize the copy number of plasmids containing the lacUV5 and tetA promoters, respectively. Repression of th...
Gespeichert in:
| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1985
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC219010/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3920207/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.1.441-444.1985 |
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