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Cloning and expression in Escherichia coli of Vibrio parahaemolyticus thermostable direct hemolysin and thermolabile hemolysin genes.

Two hemolysin genes of Vibrio parahaemolyticus WP1, a thermostable direct (TSD) hemolysin gene and a thermolabile hemolysin gene, were cloned into the pBR322 vector in Escherichia coli K-12 C600. A large amount of the TSD hemolysin produced in E. coli K-12 accumulated in the periplasmic space. The T...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Taniguchi, H, Ohta, H, Ogawa, M, Mizuguchi, Y
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1985
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218877/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3886626/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.162.2.510-515.1985
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