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Topological analysis of a plant vacuolar Na(+)/H(+) antiporter reveals a luminal C terminus that regulates antiporter cation selectivity

We conducted an analysis of the topology of AtNHX1, an Arabidopsis thaliana vacuolar Na(+)/H(+) antiporter. Several hydrophilic regions of the antiporter were tagged with a hemagglutinin epitope, and protease protection assays were conducted to determine the membrane topology of the antiporter by us...

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Detalhes bibliográficos
Main Authors: Yamaguchi, Toshio, Apse, Maris P., Shi, Huazhong, Blumwald, Eduardo
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC218788/
https://ncbi.nlm.nih.gov/pubmed/14530406
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2034966100
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