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Isolation of murine pluripotent hemopoietic stem cells
A method described to purify pluripotent hemopoietic stem cells ( PHSC ) from adult mouse bone marrow. The method consists of three separation steps. First, bone marrow cells are centrifuged in a discontinuous metrizamide gradient and simultaneously labeled with wheat germ agglutinin-fluorescein iso...
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Formato: | Artigo |
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Idioma: | Inglês |
Publicado em: |
The Rockefeller University Press
1984
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2187316/ https://ncbi.nlm.nih.gov/pubmed/6427383 |
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