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Mouse spleen lymphoblasts generated in vitro. Recovery in high yield and purity after floatation in dense bovine plasma albumin solutions
Mouse spleen lymphoblasts, stimulated to divide in vitro, acquired a low cell density and could be separated by isopycnic techniques. Cultured cells were suspended in BPA columns, rho = 1.080, and spun to equilibrium. The method was simple, fast, accomodated large numbers of cells, and was reproduci...
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| Format: | Artigo |
|---|---|
| Sprog: | Inglês |
| Udgivet: |
The Rockefeller University Press
1978
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2184489/ https://ncbi.nlm.nih.gov/pubmed/564389 |
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