Optimal conditions for transformation of Azotobacter vinelandii.

Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated imme...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Page, W J, von Tigerstrom, M
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1979
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC218056/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/479104/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.139.3.1058-1061.1979
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