Characterization and molecular cloning in Escherichia coli of a plasmid from the mollicute Spiroplasma citri.
Two plasmids, pMH1 with 7 kilobase pairs and pM41 with 8 kilobase pairs, were purified from the plant pathogen Spiroplasma citri and characterized by restriction mapping. Upon in vitro DNA recombination with plasmid pBR328 as a vector, we have cloned pMH1 in Escherichia coli. A radioactive probe obt...
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| Publicado en: | J Bacteriol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1983
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217925/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6313631/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.156.2.952-955.1983 |
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