In vivo ligation of linear DNA molecules to circular forms in the yeast Saccharomyces cerevisiae.
The yeast Saccharomyces cerevisiae was transformed with restriction endonuclease-digested (linear) DNAs containing the replication origin of the yeast 2 microns plasmid and selectable markers with efficiencies of 10(3) to 10(4), 10(3), and 10(2) to 10(3) transformants per microgram of DNA in the cas...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1983
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217746/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6307979/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.155.2.747-754.1983 |
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