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Polyethylene glycol-dependent transfection of Acholeplasma laidlawii with mycoplasma virus L2 DNA.

Phenol-extracted DNA from mycoplasma virus L2 was able to transfect Acholeplasma laidlawii in the presence of polyethylene glycol. Transfection was sensitive to DNase and was most efficient with 36% (wt/vol) polyethylene glycol 8000 and cells in logarithmic growth. Virus production by the transfecte...

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Main Authors: Sladek, T L, Maniloff, J
格式: Artigo
語言:Inglês
出版: 1983
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC217744/
https://ncbi.nlm.nih.gov/pubmed/6874642
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