Multicopy Tn10 tet plasmids confer sensitivity to induction of tet gene expression.
We inserted the Tn10 tetracycline resistance determinant (tet) into the multicopy plasmid pACYC177, and we examined the phenotype of Escherichia coli K-12 strains harboring these plasmids. In agreement with others, we find that Tn10 tet exhibits a negative gene dosage effect. Strains carrying multic...
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| Publicado en: | J Bacteriol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1983
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217722/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6307968/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.155.2.549-556.1983 |
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