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Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli.

Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. col...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Wood, D O, Atkinson, W H, Sikorski, R S, Winkler, H H
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1983
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217695/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6345509/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.155.1.412-416.1983
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