Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids.
A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes. Three...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1983
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217586/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6304005/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.154.3.1153-1161.1983 |
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