Positive-selection cloning vehicle useful for overproduction of hybrid proteins.

Plasmid pSCC31 contains the EcoRI endonuclease gene downstream from lambda pL. It does not yield transformants upon introduction into Escherichia coli unless the structural integrity of the endonuclease is destroyed. This makes it useful as a positive-selection cloning vehicle which can be employed...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Cheng, S C, Modrich, P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1983
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217560/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6302074/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.154.2.1005-1008.1983
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