High-efficiency, temperature-sensitive suppression of amber mutations in Escherichia coli.

We have constructed a high-copy-number plasmid carrying an allele of the supD gene (supD43,74). The plasmid conferred temperature-sensitive suppression of amber mutations. Strains carrying the plasmid exhibited 50 to 60% suppression at 30 degrees C but little or no suppression at 42 degrees C. After...

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Bibliographic Details
Main Authors: Zengel, J M, Lindahl, L
Format: Artigo
Language:Inglês
Published: 1981
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC217294/
https://ncbi.nlm.nih.gov/pubmed/7007324
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