Purification of penicillin-binding protein 2 of Escherichia coli.

Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin,...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Curtis, S J, Strominger, J L
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1981
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217285/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7007320/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.145.1.398-403.1981
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