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Transformation of Escherichia coli with plasmid deoxyribonucleic acid: calcium-induced binding of deoxyribonucleic acid to whole cells and to isolated membrane fractions.

Plasmid deoxyribonucleic acid (DNA) was tightly bound to cells of Escherichia coli at 0 degrees C in the presence of divalent cations. During incubation at 42 degrees C, 0.1 to 1% of this DNA became resistant to deoxyribonuclease. Deoxyribonuclease-resistant DNA binding and the ability to produce tr...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Weston, A, Brown, M G, Perkins, H R, Saunders, J R, Humphreys, G O
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1981
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC217179/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7007349/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.145.2.780-787.1981
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