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Isolation of yeast mutants defective for localization of vacuolar vital dyes

An application of flow cytometric sorting is used for isolation of Saccharomyces cerevisiae mutants that mislocalize vacuolar vital dyes. This screen is based on the ability of a lipophilic styryl compound, N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium dibromide (F...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Zheng, Bing, Wu, Jennifer N., Schober, Wendy, Lewis, Dorothy E., Vida, Thomas
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1998
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21707/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9751732/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.20.11721
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